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Sagot :
In order to elute a less polar chemical, a less polar solvent is initially utilized. A more polar solvent is then added to the column to elute the more polar compound after the less polar compound has been removed from the column.
How should the chemicals elute off the column in order?
As a result, non-polar molecules elute first. Alkyl halides, saturated hydrocarbons, unsaturated hydrocarbons, ethers, esters, ketones, amines, alcohols, phenols, acids—in that order—are typically the substances that are eluted from columns. Salts and compounds with polymers frequently do not elute.
polar-free substances. Comparatively speaking to non-polar chemicals, the polar compounds will firmly commune with the silica.
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