What is the purpose of adding 0. 1% agar to the trypticase nitrate broth when conducting the nitrate reduction test?.

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05-08-2022
Chemistry

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What is the purpose of adding 0. 1% agar to the trypticase nitrate broth when conducting the nitrate reduction test?.

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Tutorconsortium359 Tutorconsortium359 · Answer 1 · 2022-08-08T11:05:12-04:00

Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.

Nitrate Reduction test:

The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).

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