Blue-white screening is a technique for the identification of___. It relies on the ability of____to cleave lactose. Blue-white tests take advantage of a molecule called____which is similar to lactose in that it is cleaved by B-galactosidase. When cleaved, the____(same as previous) turns . If uncleaved,meaning a non- function B-gal gene, the X-gal remains____. Therefore, a____bacterial colony means the B-galactosidase gene is not-functional, and therefore there____a recombinant gene inserted into the vector.
You’ve inserted a gene for pesticide resistance into your vector. To see if it was successful, you decide to conduct a blue-white screening test. But, uh-oh you are out of X-gal! You do notice some green Slime-gal sitting on the shelf and recall that this molecule works similarly to X-gal, but instead of turning blue when cleaved, it turns green when cleaved. You carry out the test, and find that all of your colonies are green! Was your insertion of the pesticide gene successful?